ENIGMA-DTI Quality Control (QC) Protocol

Please note this protocol was developed for the ENIGMA consortium. If you are not part of the ENIGMA consortium and wish to use this protocol please register on the ENIGMA mailing list so that we can contact you regarding any updates or issues that arise relating to this protocol. You can register at http://enigma.ini.usc.edu/members/join/

If you use data generated by this protocol for non-ENIGMA projects please include the following citation for the protocol in your work:

IMPORTANT Read first!

This document contains ENIGMA-DTI-GWAS project instructions for different levels of prior genotype processing. If you have previously contributed to an ENIGMA GWAS (following their QC protocol and imputation using 1000 Genomes Phase 3 version 5 All-ethnicity reference panel) reimputation might not be required for your data set or only partially (i.e. Chr X).

If you haven’t done so yet, please also fill out the preQC form for each of your cohorts: preQCSummaryData .

Before you begin, please check carefully which case outlined below applies to you!

If you have run your QC and imputation according to the 2017 Enigma protocol (see Case 1 for links) with the X-Chromosome included in the imputation, your sample does not require any reimputing. Please do however still run the current ENIGMA-DTI QC protocol below (~1-2h) as it outputs important basic statistics needed for consortium-level QC and return the details as described. This can even be done with already QCed data as input, where both input and output should be fairly similar, thus can be used as a sanity check.

If you have run your QC and imputation according to the 2017 Enigma protocol (see Case 1 for links), excluding the X-Chromosome imputation, you might only want to rerun the X-Chromosome imputation.

Please do still run the current ENIGMA-DTI QC protocol below (~1-2h) as it outputs important basic statistics needed for consortium-level QC and return the details as described. This can even be done with already QCed data as input, where both input and output should be fairly similar, thus can be used as a sanity check.

Further, continue with the ENIGMA-DTI imputation protocol only for the X chromosome (provided by email upon request after sending the QC information), which will contain information on which steps can be skipped/adapted.

Programs required for this protocol

It is assumed that you have access to a server or computer running Linux/Unix. If this is not the case please contact us at enigma.DTIgenetics@gmail.com. Before we start, you need to download and install some required programs (which you may already have). The required programs are:

Once you have installed these software tools, ensure executable program files are on your search path:

export PATH=/path/to/plink:/path/to/R:$PATH

Please let us know if you run into problems along the way. Please address any questions to: enigma.DTIgenetics@gmail.com

Important Notes

Before starting, please ensure that your genetic data is aligned with the GRCh37/b37/hg19 genome builds.

You should start this protocol using a dataset in a binary file format for PLINK1.9, that means, in a .bed/.bim/.fam format. For more information, visit: https://www.cog-genomics.org/plink/1.9/input#bed

If your cohort has a case-control design, please assign phenotype (i.e. case-control status) in the Plink (.fam) file – coding controls or non-affected individuals as 1 and cases as 2. This information will be incorporated in the plink log files and will help with QC and troubleshooting. If in doubt, please follow the population-based instructions below.

If your cohort is population-based please code all individuals as if they were controls (1). This could be changed by e.g. changing the respective column in the .fam file with

awk '$6=1' < yourinput.fam > output.fam

Very important Please remove individuals that do not have DTI data available before running this protocol. You can do this by using the function –keep on plink1.9 and a list* of individuals, so that your genetic data is restricted to only those with also available DTI data.

plink --bfile all_genotyped_filename --keep dti_fid-iid_list.txt --make-bed --out genotyped_with_dti_filename

File naming convention for your bed/bim/fam files

We are working with the assumption that the ideal minimal final sample size of each participating cohort is approximately 100 individuals. If you know/suspect that this might not be the case for your cohort, please contact us at enigma.DTIgenetics@gmail.com

PRE IMPUTATION PIPELINE

Paste the code lines below into a terminal window or shell script, substitute the correct values where text is highlighted in red

$HOME/enigma/DTIgenetics/
mkdir -p $HOME/enigma/DTIgenetics/

Replace yourrawdata with the file name according to the convention mentioned before (no extensions needed)

cd $HOME/enigma/DTIgenetics/

Sex-check

Let’s start by checking for sex-mismatch from the phenotypic and the genetic data. First, ensure sex (as entered into the phenotype database) is encoded in the .fam file (male=1 and female=2). From the directory where your dataset (i.e. your binary plink files = yourrawdata.bim/.bed/.fam) is located, type the following commands in blue to perform a series of steps:

Perform SNP filtering and pruning for a more accurate sex-check analysis:

plink --bfile ${datafile} --mind 1 --hwe 1e-06 --geno 0.01 --maf 0.05 --make-bed --out ${datafile}_filtered
plink --bfile ${datafile}_filtered --indep-pairphase 20000 2000 0.5 --out ${datafile}_pruned
plink --bfile ${datafile}_filtered --extract ${datafile}_pruned.prune.in --make-bed --out ${datafile}_pruned

Split-X to deal with pseudo-autosomal regions

First, check if your data is already split, i.e. whether it contains an XY region.

awk '{if ($1==25) print $1}' ${datafile}_pruned.bim | wc -l

If this number is >0 there is already an XY region. In that case, skip the next plink command below

plink --bfile ${datafile}_pruned --split-x b37 no-fail --make-bed --out ${datafile}_splitX

As a rule of thumb, F estimates < 0.2 yield female calls, and F>0.8 yield male calls. Unless you have experience and reason enough to bend these thresholds slightly, we will use them as default:

plink --bfile ${datafile}_splitX --check-sex 0.2 0.8 --out ${datafile}_sexcheck

Check the log file and the ${datafile}_sexcheck file to identify if problems were detected. You can use the following command to select individuals with sex mismatch:

grep PROBLEM ${datafile}_sexcheck.sexcheck > sex.drop

The next command just extracts some important information to be sent to us as general sanity check:

wc -l sex.drop > ${datafile}_sexcheck_PROBLEM.txt

We now remove samples with sex inconsistencies between PEDSEX and SNPSEX information from your initial raw dataset:

plink --bfile ${datafile} --remove sex.drop --make-bed --out ${datafile}_QC1

For the purpose of reporting, we also count the number of SNPs on the X chromosome

awk '{if ($1=="23") print $0}' ${datafile}_QC1.bim | wc -l > snp_count_X.txt
echo "The number of SNPs on the X chromosome is:"
cat snp_count_X.txt

Check for related and duplicated samples

Starting from the dataset already cleared for sex mismatch we will check for relatedness among samples and also identify possible duplicated samples. For this analysis, we will also perform SNP filtering and pruning first:

plink --bfile ${datafile}_QC1 --mind 1 --geno 0.01 --maf 0.05 --make-bed --out ${datafile}_QC1tmp
plink --bfile ${datafile}_QC1tmp --indep-pairwise 100 5 0.2 --out ${datafile}_QC1pruned
plink --bfile ${datafile}_QC1tmp --extract ${datafile}_QC1pruned.prune.in --make-bed --out ${datafile}_QC1pruned

The following command creates a list with possible duplicates called pihat_duplicates.genome (PI_HAT > 0.9)

Now we exclude one of each pair of duplicated samples. Open the pihat_duplicates.genome file and create a secondary pihat_duplicates.txt file yourself containing only FID and IID columns of one of each pair. This can be done with e.g. using awk and extracting the first two columns of pihat_duplicates.genome file and continue with the following command:

plink --bfile ${datafile}_QC1 --remove pihat_duplicates.txt --make-bed --out ${datafile}_QC2

The next command again just extracts some information to be sent to us as a sanity check.

wc -l pihat_duplicates.txt > ${datafile}_QC1pruned_duplicates_count.txt

We will repeat this to get a rough estimate about relatedness in your sample. While not excluded this will allow us to get a better estimate of unreleated individuals for later steps.

plink --bfile ${datafile}_QC1pruned --genome --min 0.25 --max 0.9 --out pihat_relatedness

The next command again just extracts the information to be sent to us as a sanity check.

wc -l pihat_relatedness.genome > ${datafile}_QC1pruned_relatedness_count.txt

Multi-Dimensional Scaling (MDS) Protocol

Please note if you ran the MDS Protocol for ENIGMA1, 2 or 3 and you have not added any new individuals or new genotyping you do not need to re-run the MDS analysis!

If you are not using bash please change to the bash shell to run the protocol by typing:

when you are finished to go back to your usual shell (if you weren’t already in bash) type:

We will download the customized reference data from the following webpages to your working directory which we called $HOME/enigma/DTIgenetics/ where all files from the previous steps are located.

wget https://enigma.ini.usc.edu/website_downloads/ENIGMA_DTI_downloads/HapMap3/HM3_b37.bed.gz
wget https://enigma.ini.usc.edu/website_downloads/ENIGMA_DTI_downloads/HapMap3/HM3_b37.bim.gz
wget https://enigma.ini.usc.edu/website_downloads/ENIGMA_DTI_downloads/HapMap3/HM3_b37.fam.gz

Starting from the dataset generated in the previous QC step (i.e. ${datafile}_QC2), filter SNPs out from your dataset which do not meet Quality Control criteria for the MDS analysis (Minor Allele Frequency < 0.01; Genotype Call Rate < 95%; Hardy-Weinberg Equilibrium < 1x10^-6).

plink --bfile ${datafile}_QC2 --mind 1 --hwe 1e-6 --geno 0.05 --maf 0.01 --make-bed --out ${datafile}_QC2_filtered

Unzip the HM3_b37 genotypes. Prepare the HM3_b37 and the raw genotype data by extracting only SNPs that are in common between the two genotype data sets - this avoids exhausting the system memory. We are also removing the strand ambiguous SNPs from the genotyped data set to avoid strand mismatch among these SNPs. Your genotype files should be filtered to remove markers which do not satisfy the quality control criteria above.

gunzip HM3_b37.*
awk '{print $2}' HM3_b37.bim > HM3_b37.snplist.txt
plink --bfile ${datafile}_QC2_filtered --extract HM3_b37.snplist.txt --make-bed --out ${datafile}_QC2local
awk '{ if (($5=="T" && $6=="A")||($5=="A" && $6=="T")||($5=="C" && $6=="G")||($5=="G" && $6=="C")) print $2, "ambig"; else print $2 ;}' ${datafile}_QC2_filtered.bim | grep -v ambig > local.snplist.txt 
plink --bfile HM3_b37 --extract local.snplist.txt --make-bed --out HM3_b37_external

Merge the two sets of plink files – In merging the two files plink will check for strand differences. If any strand differences or multiallelic SNPs are found, plink will crash. Ignore warnings regarding different physical positions.

plink --bfile ${datafile}_QC2local --bmerge HM3_b37_external.bed HM3_b37_external.bim HM3_b37_external.fam --make-bed --out ${datafile}_QC2local_HM3b37merge

If plink crashed with a strand error (e.g. ERROR: Stopping due to mis-matching SNPs – check +/- strand?) and or multiallelic error (e.g. Error: 4 variants with 3+ alleles present), run the following two lines of alternate code:

plink --bfile ${datafile}_QC2local --flip ${datafile}_QC2local_HM3b37merge-merge.missnp --make-bed --out ${datafile}_QC2local_flipped 
plink --bfile ${datafile}_QC2local_flipped --bmerge HM3_b37_external.bed HM3_b37_external.bim HM3_b37_external.fam --make-bed --out ${datafile}_QC2local_HM3b37merge

If after the alternate step above plink still crashed with a multiallelic error (e.g. Error: 4 variants with 3+ alleles present), examine the HM3_b37merg-merge.missnp file and remove the problematic multiallelic SNPs by running the alternative code:

plink --bfile ${datafile}_QC2local_flipped --exclude  ${datafile}_QC2local_HM3b37merge-merge.missnp --make-bed --out ${datafile}_QC2local_flipped2 
plink --bfile ${datafile}_QC2local_flipped2 --bmerge HM3_b37_external.bed HM3_b37_external.bim HM3_b37_external.fam --make-bed --out ${datafile}_QC2local_HM3b37merge

Run the MDS analysis

plink --bfile ${datafile}_QC2local_HM3b37merge --cluster --mind .05 --mds-plot 10 --extract local.snplist.txt --out ${datafile}_QC2_HM3b37mds

Plot the MDS results using R

cat ${datafile}_QC2_HM3b37mds.mds | tr -s ' ' '\t' > ${datafile}_QC2_HM3b37.mds.tsv
awk 'BEGIN{OFS=","};{print $1, $2, $3, $4, $5, $6, $7}' >> ${datafile}_QC2_HM3b37mds2R.mds.csv ${datafile}_QC2_HM3b37mds.mds

From this point until the end of the section, you are working in the R statistical package. Type R to start R in unix and q() followed by n to close the R session after the plot has been created.

Please change the DATASETNAME below to match cohort specific abbreviation of your sample (e.g. BIG) and DATAFILE with the full root name used before in the shell scripts You can find out the actual name via echo $datafile in the shell and ensure you are running R from the same working directory as before (i.e. where the .csv file is located –> $HOME/enigma/DTIgenetics/${datafile}_QC2_HM3b37mds2R.mds.csv

#In R
library(calibrate)
#If you don’t have calibrate package, install it using
#install.packages("calibrate")

#first we create an additional column holding the respective ancestry labels

mds.cluster$POP[which(mds.cluster$FID == "CEU")] <- "CEU"; 
mds.cluster$POP[which(mds.cluster$FID == "CHB")] <- "CHB"; 
mds.cluster$POP[which(mds.cluster$FID == "YRI")] <- "YRI"; 
mds.cluster$POP[which(mds.cluster$FID == "TSI")] <- "TSI"; 
mds.cluster$POP[which(mds.cluster$FID == "JPT")] <- "JPT"; 
mds.cluster$POP[which(mds.cluster$FID == "CHD")] <- "CHD"; 
mds.cluster$POP[which(mds.cluster$FID == "MEX")] <- "MEX"; 
mds.cluster$POP[which(mds.cluster$FID == "GIH")] <- "GIH"; 
mds.cluster$POP[which(mds.cluster$FID == "ASW")] <- "ASW"; 
mds.cluster$POP[which(mds.cluster$FID == "LWK")] <- "LWK"; 
mds.cluster$POP[which(mds.cluster$FID == "MKK")] <- "MKK";

#get the colors for plotting
colors=rep("red",length(mds.cluster$C1)); 
colors[which(mds.cluster$POP == "CEU")] <- "lightblue"; 
colors[which(mds.cluster$POP == "CHB")] <- "brown"; 
colors[which(mds.cluster$POP == "YRI")] <- "yellow"; 
colors[which(mds.cluster$POP == "TSI")] <- "green"; 
colors[which(mds.cluster$POP == "JPT")] <- "purple"; 
colors[which(mds.cluster$POP == "CHD")] <- "orange"; 
colors[which(mds.cluster$POP == "MEX")] <- "grey50"; 
colors[which(mds.cluster$POP == "GIH")] <- "black"; 
colors[which(mds.cluster$POP == "ASW")] <- "darkolivegreen"; 
colors[which(mds.cluster$POP == "LWK")] <- "magenta"; 
colors[which(mds.cluster$POP == "MKK")] <- "darkblue";

#Please note - the plot will directly be saved as a PDF in your current working directory under the above specified name 
#and does not appear in the PLOT window
plot(rev(mds.cluster$C2), rev(mds.cluster$C1), col=rev(colors), ylab="Dimension 1", xlab="Dimension 2",pch=20) 
minor.tick(nx = 5, ny = 5,   # Ticks density
         tick.ratio = 0.5) # Ticks size

legend("bottomleft", c("DATASETNAME

", "CEU", "CHB", "YRI", "TSI", "JPT", "CHD", "MEX", "GIH", "ASW","LWK", "MKK"), fill=c("red", "lightblue", "brown", "yellow", "green", "purple", "orange", "grey50", "black", "darkolivegreen", "magenta", "darkblue"))

dev.off(); 
#If you want to know the subject ID label of each sample on the graph, you can label your sample points by uncommenting the commands below.
#This is optional and you can choose not to do this if you are worried about patient information being sent; when you send us your MDS plot please make sure the subject ID labels are NOT on the graph.
#POPlabels <- c("CEU", "CHB", "YRI", "TSI", "JPT", "CHD", "MEX", "GIH", "ASW","LWK", "MKK"); 
#textxy(mds.cluster[which(!(mds.cluster$POP %in% POPlabels)), "C2"], mds.cluster[which(!(mds.cluster$POP %in% POPlabels)), "C1"], mds.cluster[which(!(mds.cluster$POP %in% POPlabels)), "IID"])

Identify Ancestry Outliers

For predominantly European ancestry cohorts, we now exclude individuals below cut-off thresholds. These thresholds are not fixed but will need to be determined based on the visualization/plot of the two dimensions you just created. Please have a good look at the two examples above - depending on your sample please change the cut-off threshold marked in red below.

For predominantly other ancestry cohorts, the threshold should be determined according to the respective cohort cluster.

# First we grab your specific sample and create a column where you can mark outliers
#--> change “DATASETNAME” to your sample specific cohort abbreviation

MDS_mySample$outlier  <- 0

# calculate outliers - first a threshold example for the g1000mix, a quite homogenous sample

# sort sample specific outliers /Europeans, just grab the FID and IID column for later use
MDS_mySample_outliers<- MDS_mySample[which(MDS_mySample$outlier == 1),1:2]
MDS_mySample_european<- MDS_mySample[which(MDS_mySample$outlier == 0 ),1:2]
#For further plotting we also grab the full reference details again and combine them with the full EU information in your sample
#First let's get the reference - we get everything not part of your dataset

#combine this with all Europeans (or respective other ancestry cohort)
MDS_mySample_eur_plus_ref <- rbind(MDS_ref_eur, mds.cluster[which (mds.cluster$IID %in% MDS_mySample_european$IID ),])

# repeat the colours again for the updated dataframe and then plot - note again: plot is directly saved 
colors=rep("red",length(MDS_mySample_eur_plus_ref$C1)); 
colors[which(MDS_mySample_eur_plus_ref$POP == "CEU")] <- "lightblue"; 
colors[which(MDS_mySample_eur_plus_ref$POP == "CHB")] <- "brown"; 
colors[which(MDS_mySample_eur_plus_ref$POP == "YRI")] <- "yellow"; 
colors[which(MDS_mySample_eur_plus_refr$POP == "TSI")] <- "green"; 
colors[which(MDS_mySample_eur_plus_ref$POP == "JPT")] <- "purple"; 
colors[which(MDS_mySample_eur_plus_ref$POP == "CHD")] <- "orange"; 
colors[which(MDS_mySample_eur_plus_ref$POP == "MEX")] <- "grey50"; 
colors[which(MDS_mySample_eur_plus_ref$POP == "GIH")] <- "black"; 
colors[which(MDS_mySample_eur_plus_ref$POP == "ASW")] <- "darkolivegreen"; 
colors[which(MDS_mySample_eur_plus_ref$POP == "LWK")] <- "magenta"; 
colors[which(MDS_mySample_eur_plus_ref$POP == "MKK")] <- "darkblue";

plot(rev(MDS_mySample_eur_plus_ref$C2), rev(MDS_mySample_eur_plus_ref$C1), col=rev(colors), ylab="Dimension 1", xlab="Dimension 2", pch=20)

legend("bottomleft", c(“DATASETNAME”, "CEU", "CHB", "YRI", "TSI", "JPT", "CHD", "MEX", "GIH", "ASW","LWK",

"MKK"), fill=c("red", "lightblue", "brown", "yellow", "green", "purple", "orange", "grey50", "black", "darkolivegreen", "magenta",

"darkblue"))

# save files - don't forget to replace DATAFILE with your cohort specific abbreviation 
#let's get the outliers in your sample
write.table(MDS_mySample_outliers, "DATAFILE_pop_strat_mds.outlier.txt", sep="\t", quote=FALSE, row.names=FALSE)
#let's get the europeans in your sample 
write.table(MDS_mySample_european, "DATAFILE_pop_strat_mds.eur.txt", sep="\t", quote=FALSE, row.names=FALSE)
# quit R 
q()

Your output will now have outliers removed that are not part of the European (or respective ancestry of your cohort) cluster: In case of the first included graph the output contains only the individuals from your sample within the dotted circle.

Back in the shell, we exclude outliers from your actual bfile and use this QC3 file for subsequent steps:

plink --bfile ${datafile}_QC2  --keep ${datafile}_pop_strat_mds.eur.txt --make-bed --out ${datafile}_QC3

Get genetic principal components in ancestry homogenous group

plink --bfile ${datafile}_QC3 --pca --extract local.snplist.txt --out ${datafile}_PCACovariates

This extracts by default 20 PCs, which can be found in the .eigenvec file. More info on how many PCs to include as covariates will follow in the association protocol but to get a feeling you can create a scree plot and see how many PC’s are needed to explain most of the variance.

library(data.table)
library(ggplot2)

#read in the eigenvalue file
eigv <- fread("${datafile}_PCACovariates.eigenval")
#calculate variance explained
var_explained <- (eigv/(sum(eigv))*100
#plot the scree plot
qplot(c(1:20), var_explained$V1) + 
geom_line() + 
xlab("Principal Component") + 
ylab("Variance Explained") +
ggtitle("Scree Plot") +
ylim(0, 100)

Cohort QC summary data

Using the ${datafile}, ${datafile}_QC2_filtered and ${datafile}_QC3 file, respectively, please run the bash codes below to obtain an overview of the cohort stats pre- and post-QC:

Pre-QC subject numbers

echo "COHORTNAME N_CONTROLS N_CASES N_CONTROLS_M N_CONTROLS_F N_CASES_M N_CASES_F PROP_CONTROLS_F PROP_CONTROLS_M PROP_CASES_F PROP_CASES_M" > ${datafile}_basic_stats_preQC.txt
echo -n "${datafile} " >> ${datafile}_basic_stats_preQC.txt
#How many controls/unaffected are in the cohort? 
N_CONTROLS=`awk '{if ($6==1) print $0}' ${datafile}.fam | wc -l`
echo -n "$N_CONTROLS " >> ${datafile}_basic_stats_preQC.txt
#How many patients are in the cohort? 
N_CASES=`awk '{if ($6==2) print $0}' ${datafile}.fam | wc -l`
echo -n "$N_CASES " >> ${datafile}_basic_stats_preQC.txt
#What is the absolute number of males among controls/unaffected? 
N_CONTROLS_M=`awk '{if ($6==1 && $5==1) print $0}' ${datafile}.fam | wc -l`
echo -n "$N_CONTROLS_M " >> ${datafile}_basic_stats_preQC.txt
#What is the absolute number of females among controls/unaffected? 
N_CONTROLS_F=`awk '{if ($6==1 && $5==2) print $0}' ${datafile}.fam | wc -l`
echo -n "$N_CONTROLS_F " >> ${datafile}_basic_stats_preQC.txt
#What is the absolute number of males among patients? 
N_CASES_M=`awk '{if ($6==2 && $5==1) print $0}' ${datafile}.fam | wc -l`
echo -n "$N_CASES_M " >> ${datafile}_basic_stats_preQC.txt
#What is the absolute number of females among patients? 
N_CASES_F=`awk '{if ($6==2 && $5==2) print $0}' ${datafile}.fam | wc -l`
echo -n "$N_CASES_F " >> ${datafile}_basic_stats_preQC.txt
#What is the proportion of females among controls/unaffected?
PROP_CONTROLS_F=`echo "$N_CONTROLS_F/$N_CONTROLS" | bc -l`
#What is the proportion of males among controls/unaffected?
PROP_CONTROLS_M=`echo "$N_CONTROLS_M/$N_CONTROLS" | bc -l`
#What is the proportion of females among patients? 
PROP_CASES_F=`if (($N_CASES == 0)); then echo 0; else echo "$N_CASES_F/($N_CASES_F+$N_CASES_M)" | bc -l; fi`
#What is the proportion of males among patients? 
PROP_CASES_M=`if (($N_CASES == 0)); then echo 0; else echo "$N_CASES_M/($N_CASES_F+$N_CASES_M)" | bc -l; fi`
echo -n "$PROP_CONTROLS_F $PROP_CONTROLS_M $PROP_CASES_F $PROP_CASES_M " >> ${datafile}_basic_stats_preQC.txt

SNP level QC

echo "COHORTNAME N_SNPs_preQC N_samples_preQC SNPs_removed_>missingnessT Samples_removed_>missingnessT SNPs_removed_<MAFt SNPs_removed_HWE Samples_removed_MDS_outliers N_SNPs_postQC N_samples_postQC" > ${datafile}_qc_summary.txt
echo -n "${datafile} " >> ${datafile}_qc_summary.txt 
NLINES=`wc -l ${datafile}.bim | awk '{print $1}'`
echo -n $NLINES" " >> ${datafile}_qc_summary.txt
NLINES=`wc -l ${datafile}.fam | awk '{print $1}'`
echo -n $NLINES" " >> ${datafile}_qc_summary.txt
qc_VAR=$(grep "variants removed due to missing genotype data (--geno)" ${datafile}_QC2_filtered.log | awk '{printf $1}')
if [ -z "$qc_VAR" ];then echo -n "NaN"" " >> ${datafile}_qc_summary.txt; else echo -n "$qc_VAR"" " >> ${datafile}_qc_summary.txt;fi
qc_VAR=$(grep "people removed due to missing genotype data (--mind)" ${datafile}_QC2_filtered.log | awk '{printf $1}')
if [ -z "$qc_VAR" ];then echo -n "NaN"" " >> ${datafile}_qc_summary.txt; else echo -n "$qc_VAR"" " >> ${datafile}_qc_summary.txt;fi
qc_VAR=$(grep "variants removed due to minor allele threshold(s)" ${datafile}_QC2_filtered.log | awk '{printf $1}')
if [ -z "$qc_VAR" ];then echo -n "NaN"" " >> ${datafile}_qc_summary.txt; else echo -n "$qc_VAR"" " >> ${datafile}_qc_summary.txt;fi
qc_VAR=$(grep "variants removed due to Hardy-Weinberg exact test" ${datafile}_QC2_filtered.log | awk '{printf $2}')
if [ -z "$qc_VAR" ];then echo -n "NaN"" " >> ${datafile}_qc_summary.txt; else echo -n "$qc_VAR"" " >> ${datafile}_qc_summary.txt;fi
NLINES2=`wc -l ${datafile}_QC2.bim | awk '{print $1}'`
NLINES3=`wc -l ${datafile}_QC3.bim | awk '{print $1}'`
N_excluded_MDS_outliers=`echo "$NLINES2-$NLINES3" | bc -l`
echo -n $N_excluded_MDS_outliers" " >> ${datafile}_qc_summary.txt
NLINES=`wc -l ${datafile}_QC3.bim | awk '{print $1}'`
echo -n $NLINES" " >> ${datafile}_qc_summary.txt
NLINES=`wc -l ${datafile}_QC3.fam | awk '{print $1}'`
echo -n $NLINES" " >> ${datafile}_qc_summary.txt

Post-QC subject numbers

echo "COHORTNAME N_CONTROLS N_CASES N_CONTROLS_M N_CONTROLS_F N_CASES_M N_CASES_F PROP_CONTROLS_F PROP_CONTROLS_M PROP_CASES_F PROP_CASES_M" > ${datafile}_basic_stats_postQC.txt
echo -n "${datafile} " >> ${datafile}_basic_stats_postQC.txt
#How many controls/unaffected are in the cohort? 
N_CONTROLS=`awk '{if ($6==1) print $0}' ${datafile}_QC3.fam | wc -l`
echo -n "$N_CONTROLS " >> ${datafile}_basic_stats_postQC.txt
#How many patients are in the cohort? 
N_CASES=`awk '{if ($6==2) print $0}' ${datafile}_QC3.fam | wc -l`
echo -n "$N_CASES " >> ${datafile}_basic_stats_postQC.txt
#What is the absolute number of males among controls/unaffected? 
N_CONTROLS_M=`awk '{if ($6==1 && $5==1) print $0}' ${datafile}_QC3.fam | wc -l`
echo -n "$N_CONTROLS_M " >> ${datafile}_basic_stats_postQC.txt
#What is the absolute number of females among controls/unaffected? 
N_CONTROLS_F=`awk '{if ($6==1 && $5==2) print $0}' ${datafile}_QC3.fam | wc -l`
echo -n "$N_CONTROLS_F " >> ${datafile}_basic_stats_postQC.txt
#What is the absolute number of males among patients? 
N_CASES_M=`awk '{if ($6==2 && $5==1) print $0}' ${datafile}_QC3.fam | wc -l`
echo -n "$N_CASES_M " >> ${datafile}_basic_stats_postQC.txt
#What is the absolute number of females among patients? 
N_CASES_F=`awk '{if ($6==2 && $5==2) print $0}' ${datafile}_QC3.fam | wc -l`
echo -n "$N_CASES_F " >> ${datafile}_basic_stats_postQC.txt
#What is the proportion of females among controls/unaffected?
PROP_CONTROLS_F=`echo "$N_CONTROLS_F/$N_CONTROLS" | bc -l`
#What is the proportion of males among controls/unaffected?
PROP_CONTROLS_M=`echo "$N_CONTROLS_M/$N_CONTROLS" | bc -l`
#What is the proportion of females among patients? 
PROP_CASES_F=`if (($N_CASES == 0)); then echo 0; else echo "$N_CASES_F/($N_CASES_F+$N_CASES_M)" | bc -l; fi`
#What is the proportion of males among patients? 
PROP_CASES_M=`if (($N_CASES == 0)); then echo 0; else echo "$N_CASES_M/($N_CASES_F+$N_CASES_M)" |  bc -l; fi`
echo -n "$PROP_CONTROLS_F $PROP_CONTROLS_M $PROP_CASES_F $PROP_CASES_M " >> ${datafile}_basic_stats_postQC.txt

Information about data transfer

Please send the following information and files to The ENIGMA-DTI Genetics Support Team enigma.DTIgenetics@gmail.com for checking before running the imputation protocol and fill out the postQC form: postQCSummaryData.

We will get back to you shortly with any questions or to give the go-ahead for the imputation.

The following lines of bash code gather and compress the requested files into a single zip archive:

mkdir output_final
cp *.pdf *.log ${datafile}_QC1pruned_duplicates_count.txt ${datafile}_QC1pruned_relatedness_count.txt ${datafile}_sexcheck_PROBLEM.txt ${datafile}_basic_stats_postQC.txt ${datafile}_qc_summary.txt ${datafile}_basic_stats_preQC.txt snp_count_X.txt output_final/

The line below checks if the expected amount of files have been moved. If you ran the whole pipeline there should be 27 files - 18 PLINK log files and the 9 files specified above.

ENIGMA-DTI Quality Control (QC) Protocol - Pre Imputation

ENIGMA QC - Pre Imputation Protocol

IMPORTANT Read first!